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A practical guide to free tools for analyzing Sanger sequencing chromatograms — from .ab1 viewers to CLI variant callers.
Bioinformatics
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t.test() and aov() replace ten minutes of clicking in GraphPad Prism. Here is how to run t-tests, ANOVA, and post-hoc comparisons on your qPCR data — with code you can actually reproduce.
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AlphaFold3 can predict your protein's 3D structure from a sequence in minutes — for free. Here's how it works, how to use the server, and what those confidence scores actually mean.
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ggplot2 turns your summarized qPCR data into publication-ready figures in a dozen lines of code. Here is how to build dot plots, bar charts, and multi-panel figures — and export them at 300 dpi without touching GraphPad.
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dplyr turns data wrangling from a click-and-copy nightmare into five readable lines of code. Here is how to filter, summarize, and reshape your qPCR data without touching Excel.