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Your qPCR run is done and you have a column of Ct values. Here is how to turn them into fold changes with the ΔΔCt method, worked by hand, in a spreadsheet and in R, plus the mistakes that get qPCR figures sent back by reviewers.
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- bioinformatics (33)
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A practical guide to designing PCR primers that work — covering the rules every bench scientist should know, plus an honest comparison of the best free web tools.
