Crispr

Your amplicon FASTQ files are back from the sequencer, and the default CRISPResso2 numbers are already lying to you about your HDR and base editing rates. Here is how to run CRISPResso2 properly from raw paired-end reads to publication-ready allele tables.

For thirteen years, every CRISPR tool in your freezer was mined from a microbe. In 2025 and 2026, two papers changed that — AI-generated editors that work in human cells and, in one case, outperform the natural enzyme. Here is what the data actually shows, and what it means for your bench.

Short-read amplicon NGS is the gold standard for CRISPR indel detection — but it has a blind spot for structural variants, large deletions, and complex rearrangements. Long-read sequencing from Oxford Nanopore or PacBio fills that gap. Here is when it matters and how to actually run the analysis.

An overview and comparison of laboratory methods for finding CRISPR off-target editing. We compare GUIDE-seq, CIRCLE-seq, Digenome-seq, and DISCOVER-seq to help you choose the right validation tool.

A practical guide to designing a cost-effective and accurate amplicon next-generation sequencing (NGS) strategy to quantify CRISPR editing efficiency and characterises indels.