Crispr

An overview and comparison of laboratory methods for finding CRISPR off-target editing. We compare GUIDE-seq, CIRCLE-seq, Digenome-seq, and DISCOVER-seq to help you choose the right validation tool.

A practical guide to designing a cost-effective and accurate amplicon next-generation sequencing (NGS) strategy to quantify CRISPR editing efficiency and characterises indels.

T7E1 tells you if CRISPR worked. TIDE and ICE tell you how well. This post explains how both tools deconvolve your Sanger sequencing trace into a real editing percentage — no NGS required.

Mismatch-cleavage assays are the quickest and cheapest way to see if your CRISPR edit actually worked. This guide breaks down the mechanism of T7E1 and Surveyor nucleases, why the "denature and re-anneal" step is critical, and how to turn gel bands into an editing percentage.

You’ve made your edit, but how much of it is actually there? This post compares the three most common ways to measure CRISPR on-target efficiency—from the "quick and dirty" T7E1 gel assay to the high-resolution precision of Amplicon NGS.