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If you know CRISPR exists but aren't sure how to design your experiment or analyze your data, this 22-post series takes you through the whole thing — from Cas9 mechanism to running NGS pipelines.
Crispr
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Before you design your first CRISPR experiment, you need to understand what Cas9 is actually doing to your DNA — and what happens after the cut. This post covers the mechanism, PAM sites, and why repair pathway choice determines your experimental outcome.
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An honest comparison of the best guide RNA design platforms — scoring on ease of use, off-target prediction, and species support.