Gene-editing

Base editing converts a single nucleotide directly — no double-strand break, no donor template — with efficiencies that routinely outperform HDR in primary and non-dividing cells. This post covers how CBE and ABE work, the editing window, bystander edits, and when to choose base editing over Cas9+HDR or prime editing.

SpCas9 dominates CRISPR, but it has real constraints. Cas12a uses a T-rich PAM, cuts in a staggered fashion, processes its own guide RNAs, and opens up AT-rich targets Cas9 cannot reach. This post covers the full Cas12 family, the Cas12a mechanism, and a complete head-to-head comparison to help you choose the right tool.

If you know CRISPR exists but aren't sure how to design your experiment or analyze your data, this 22-post series takes you through the whole thing — from Cas9 mechanism to running NGS pipelines.

Before you design your first CRISPR experiment, you need to understand what Cas9 is actually doing to your DNA — and what happens after the cut. This post covers the mechanism, PAM sites, and why repair pathway choice determines your experimental outcome.

An honest comparison of the best guide RNA design platforms — scoring on ease of use, off-target prediction, and species support.