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pegRNA design has five parameters that each affect prime editing efficiency. Here are the principles behind PBS length, RT template, nick distance, and secondary structure — then how to apply them in PrimeDesign.
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- Published on
A practical guide to free tools for analyzing Sanger sequencing chromatograms — from .ab1 viewers to CLI variant callers.- Published on
AlphaFold3 can predict your protein's 3D structure from a sequence in minutes — for free. Here's how it works, how to use the server, and what those confidence scores actually mean.- Published on
A practical guide to designing PCR primers that work — covering the rules every bench scientist should know, plus an honest comparison of the best free web tools.- Published on
An in-depth look at CellPAINT — the free, scientifically accurate molecular painting tool from Scripps — and how it compares to BioRender, ChimeraX, PyMOL, and other illustration platforms.
