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Six steps from raw CSV to a publication-ready figure with significance bars. A complete, copy-paste-ready R workflow using dplyr, ggplot2, and ggsignif — built on the qPCR dataset from Arc 1.
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Prime editing writes any small edit directly into your genome — transversions, insertions, deletions — without cutting both DNA strands. This post covers the mechanism, PE2/PE3/PE7 variants, and when to choose prime editing over base editing or HDR.
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A practical guide to free tools for analyzing Sanger sequencing chromatograms — from .ab1 viewers to CLI variant callers.
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t.test() and aov() replace ten minutes of clicking in GraphPad Prism. Here is how to run t-tests, ANOVA, and post-hoc comparisons on your qPCR data — with code you can actually reproduce.
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Base editing converts a single nucleotide directly — no double-strand break, no donor template — with efficiencies that routinely outperform HDR in primary and non-dividing cells. This post covers how CBE and ABE work, the editing window, bystander edits, and when to choose base editing over Cas9+HDR or prime editing.