Bioinformatics

GEO has thousands of published RNA-seq datasets — including the one from that paper you just read. This post shows you how to pull any GEO dataset into R with GEOquery, extract the count matrix and sample metadata, and save both as CSVs for downstream analysis.

Six steps from raw CSV to a publication-ready figure with significance bars. A complete, copy-paste-ready R workflow using dplyr, ggplot2, and ggsignif — built on the qPCR dataset from Arc 1.

A practical guide to free tools for analyzing Sanger sequencing chromatograms — from .ab1 viewers to CLI variant callers.

t.test() and aov() replace ten minutes of clicking in GraphPad Prism. Here is how to run t-tests, ANOVA, and post-hoc comparisons on your qPCR data — with code you can actually reproduce.

AlphaFold3 can predict your protein's 3D structure from a sequence in minutes — for free. Here's how it works, how to use the server, and what those confidence scores actually mean.